tag:blogger.com,1999:blog-2028565150475419281.post8559064321392329520..comments2024-01-23T15:18:52.111-05:00Comments on Trichopterology: Relaxing insects.Kaihttp://www.blogger.com/profile/15709111344826156855noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-2028565150475419281.post-17866037308797784632011-11-28T14:20:01.314-05:002011-11-28T14:20:01.314-05:00Critical point drying sounds awesome, but also rea...Critical point drying sounds awesome, but also really expensive, Morgan. I'd love to have a system I could use in a small lab with a fume hood.Kaihttps://www.blogger.com/profile/15709111344826156855noreply@blogger.comtag:blogger.com,1999:blog-2028565150475419281.post-24707811613966871872011-11-27T21:03:35.756-05:002011-11-27T21:03:35.756-05:00At the University of Guelph Insect Collection we u...At the University of Guelph Insect Collection we use a sequential ethyl acetate bath for the medium-large soft bodied insects (Diptera, aquatics, etc) and Critical Point Drying (CPD) for the small and really small things. The CPD uses liquid carbon dioxide held in a high pressure chamber to force the 100% ethanol out of the specimen in a flash, leaving the specimens in a near perfect state (haven't tried it with a honey bee, so no clue how the hairs would turn out in comparison). Takes about a week to transfer the specimens through the ethanol ladder, but we can dry 16 vials of insects/CPD run, so it's pretty efficient.Morgan Jacksonhttps://www.blogger.com/profile/04693193551329558541noreply@blogger.comtag:blogger.com,1999:blog-2028565150475419281.post-3983740555969045532011-11-09T17:13:02.178-05:002011-11-09T17:13:02.178-05:00Thanks Chris! I've heard that all the water ha...Thanks Chris! I've heard that all the water has to be removed from the specimens prior to soaking because it makes ammonium gas. This is probably in addition to the other health hazards. Dr. Peter Adler has told me that it works so well, you can run a honey bee through it and the hair comes out unmatted. I'd love to try it sometime, but given I don't have a fume hood right now I just can't risk it.<br /><br />~Kai ZL BuringtonKaihttps://www.blogger.com/profile/15709111344826156855noreply@blogger.comtag:blogger.com,1999:blog-2028565150475419281.post-79297887987020404502011-11-07T22:47:06.704-05:002011-11-07T22:47:06.704-05:00Kai, we use HMDS at the Lyman Entomological museum...Kai, we use HMDS at the Lyman Entomological museum to dry all of our small diptera (i.e acalyptrates and nematocerans) and works really well. The only special equipment needed is a fumehood as the chemical is incredibly volatile and has an associated health hazard (can't remember exactly what). More info here: http://www.cdfa.ca.gov/plant/ppd/entomology/HMDS.html<br /> <br />For larger diptera/insects you can use a progression through 100% EtOH and then into 50/50 ethyl acetate and EtOH and a final soak in 100% Ethyl actete (similar to this method http://pubs.esc-sec.ca/doi/abs/10.4039/Ent9869-1). <br />Hope that helps.<br /><br />Chris BorkentAnonymousnoreply@blogger.com